NUTRITIONAL CHEMISTRY AND GENETIC MANUIPULATIONS OF LATHYRUS PEA

2,500.00

Author: CHAVAN,U.D.

ISBN: 9788192229089

Binding:  Hardback

Year: 2014

Publisher: Agri BioVet Press

SKU: 9788192229089 Category: Tags: , , , , ,

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Contents

Part-I. Nutritional Chemistry of Lathyrus Pea

1. History of Lathyrus pea, 2. Botany of Lathyrus species, 3. Chemical composition of beach pea plant parts, 4. Antinutrients, 5. Tannins and phenolic acids, 6. Effect of different solvents on the recovery of condensed tannins from beach pea, green pea and grass peas, 7. Phytic acid, 8. Chlorophyll, 9. Oligosaccharides, 10. Saponins, 11. Nutrient distribution in air classified fractions of cotyledons and hulls of beach pea, 12. Seed coat structure of beach pea, 13. Antioxidant activity of phenolic fractions of beach pea hull extract, 14. Effect of different solvent extractions on phenolic compounds, tannins and sugars of beach pea seeds, 15. Effects of methanol-ammonia-water/hexane extraction on the nutrients and antinutrients of beach pea and grass pea, 16. Dehulling, 17. Extraction with chemicals, 18. Physico-chemical properties of pea seed proteins, 19. Surface topography of protein isolates and protein fractions, 20. Polyacrylamide gel electrophoresis (PAGE) of protein isolates and protein fractions from beach pea, green pea and grass pea, 21. Lathyrus pea proteins, 22. Functional properties of food proteins, 23. Other functional properties, 24. In-vitro protein digestibility, 25. Starch, 26. Differential scanning calorimetry (DSC), 27. Pasting characteristics, 28. Soaking and cooking effect on nutritional quality, 29. Effect of thermal treatment on nutritional quality, 30. Summary, 31. References

Part-II. Genetic Manipulation of Lathyrus Pea

1. Plant Secondary Metabolites, 2. Economic significance of secondary metabolites in plants, 3. Non-protein amino – acids in plant species (Non protein amino acids in Vicia and Lathyrus), 4. Distribution and economical importance of Lathyrus, 5. Human neurolathyrism, 6. Molecular mechanisms underlying the induction of neurolathyrism, 7. P-ODAP and neurolathyrism, 8. Neurolathyrism – a socio-medical problem, 9. Metabolic pathway of the biosynthesis of P-ODAP, 10. Developmental variation in the biosynthesis of P-ODAP in L.sativus, 11. Gene regulation of secondary metabolism in plants, 12. Methods available for the genetic manipulation of P-ODAP biosynthesis in Lathyrus sativus, 13. The genetic manipulation of L. sativus using oxalate catabolling genes, 14. Preliminary comparison of the genomic DNA by restriction endonuclease analysis, 15. Construction and screening of cDNA library, 16. Construction of cDNA library (synthesis of cDNA), 17. Screening of the cDNA library, 18. Reprobing the amplified inserts with Cysteine synthase, 19. Sub-cloning of the cysteine synthase sequences, 20. Cellular oxalate decarboxylation -a possible method for the prevention of neurotoxin biosynthesis, 21. Isolation and cloning of Oxalyl CoA decarboxylase gene, 22. Search for Oxalyl CoA decarboxylase genes in plant species, 23. Construction and screening of a cDNA library of L.tingibanus for genes controlling oxalate decarboxylase activity, 24. Cloning of cDNA, 25. Screening the cDNA library of L. tingtbanus for oxalyl decarboxylase gene sequences, 26. Sequencing of the sub-cloned cDNA clones and PCR amplified fragments, 27. Non protein amino acids and P-ODAP, 28. The biosynthesis of P-ODAP in L. sativus, 29. Restriction enzyme analysis of the genomic DNA of Lothyrus species, 30. Construction and screening of cDNA library, 31. Isolation and cloning of oxalyl CoA decarboxylase gene from O. formigenes by PCR amplification, 32. Survey for oxalyl CoA decarboxylase genes in plant species, 33. Screening of the cDNA library of L.tingitanus to identify cDNA clones homologous to oxalyl decarboxylase gene sequences, 34. Plan of further research, 35. Summary and Conclusions, 36. References 37. APPENDIX – I Tissue culture, 38. APPENDIX – II Gene construction

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